Author(s):
Pinto, Miguel 
; Louro, Henriqueta ; Costa, Pedro ; Costa, Maria Helena ; Caeiro, Sandra ; Silva, Maria João
Date: 2011
Persistent ID: http://hdl.handle.net/10400.18/292
Origin: Repositório Científico do Instituto Nacional de Saúde
Subject(s): Genotoxicidade Ambiental
Description
Previous studies have shown that the river Sado Estuary (W Portugal) is highly susceptible to contamination due to
anthropogenic activity. In fact, sizable concentrations of metals, pesticides and polycyclic aromatic hydrocarbons have
been measured in sediments from various points of the estuary. Those compounds may be absorbed by aquatic species,
making it a public health issue when entering the human food chain. The purpose of this study was to assess the cytotoxic
and genotoxic potential of sediment extracts in a human cell line, through the neutral red uptake assay and the alkaline
comet assay, respectively. Three sediment samples were collected: two in potentially contaminated sites (samples C and F,
Sado Estuary) and one in a putative control station (sample M, Mira Estuary). The overall contaminants were extracted
from the three samples. HepG2 cells were exposed for 48h to serial dilutions of each extract (ranging from 0.01 to 2μl). A
dose-related decrease in cell viability was observed for samples F and M (from 0.1 and 0.5μl, respectively, up to 2μl),
indicating a toxic effect caused by the complex contaminants mixtures. However, no genotoxicity could be observed for any
sample in the experimental conditions used. Further studies are underway with different exposure times and different
endpoints for a more complete assessment of the extracts' genotoxic properties.
