Sample sonication after trichloroacetic acid precipitation increases protein re...

• Autor(es): Manadas, Bruno J. ; Vougas, Konstantinos ; Fountoulakis, Michael ; Duarte, Carlos B.
• Data: 2006
• Identificador Persistente: http://hdl.handle.net/10316/8319
• Origem: Estudo Geral - Universidade de Coimbra
• Tipo de Documento: Artigo
• Idioma: Inglês

Descrição

Protein precipitation with TCA followed by acetone washing is frequently used to clean samples before 2-DE. However, the difficulty in solubilizing TCA-precipitated proteins causes some variability in 2-D gels and makes it difficult to detect some proteins. In this work we show that sonication of the samples, after TCA precipitation followed by elution in sample buffer, increases total protein recovery, and improves reproducibility and matching ratios between gels when analyzed by specialized software. http://dx.doi.org/10.1002/elps.200500757