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Sample sonication after trichloroacetic acid precipitation increases protein re...
Manadas, Bruno J.; Vougas, Konstantinos; Fountoulakis, Michael; Duarte, Carlos B.Protein precipitation with TCA followed by acetone washing is frequently used to clean samples before 2-DE. However, the difficulty in solubilizing TCA-precipitated proteins causes some variability in 2-D gels and makes it difficult to detect some proteins. In this work we show that sonication of the samples, after TCA precipitation followed by elution in sample buffer, increases total protein recovery, and imp...
Intracellular signaling mechanisms in photodynamic therapy
Almeida, Ramiro D.; Manadas, Bruno J.; Carvalho, Arsélio P.; Duarte, Carlos B.In photodynamic therapy (PDT) a sensitizer, light and oxygen are used to induce death of tumor cells and in the treatment of certain noncancerous conditions. Cell death in PDT may occur by apoptosis or by necrosis, depending on the sensitizer, on the PDT dose and on the cell genotype. Some sensitizers that have been used in PDT are accumulated in the mitochondria, and this may explain their efficiency in induci...
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