Encontrados 32 documentos, a visualizar página 1 de 4
BDNF-induced local protein synthesis and synaptic plasticity
Leal, Graciano; Comprido, Diogo; Duarte, Carlos B.Brain-derived neurotrophic factor (BDNF) is an important regulator of synaptic transmission and longterm potentiation (LTP) in the hippocampus and in other brain regions, playing a role in the formation of certain forms of memory. The effects of BDNF in LTP are mediated by TrkB (tropomyosin-related kinase B) receptors, which are known to be coupled to the activation of the Ras/ERK, phosphatidylinositol 3-kinase...
Role of the ubiquitin–proteasome system in brain ischemia: Friend or foe?
Caldeira, Margarida V.; Salazar, Ivan L.; Cursio, Michele; Canzoniero, Lorella M. T.; Duarte, Carlos B.The ubiquitin–proteasome system (UPS) is a catalytic machinery that targets numerous cellular proteins for degradation, thus being essential to control a wide range of basic cellular processes and cell survival. Degradation of intracellular proteins via the UPS is a tightly regulated process initiated by tagging a target protein with a specific ubiquitin chain. Neurons are particularly vulnerable to any change ...
Characterization of alternatively spliced isoforms of AMPA receptor subunits en...
Gomes, André R.; Ferreira, Joana S.; Paternain, Ana V.; Lerma, Juan; Duarte, Carlos B.; Carvalho, Ana LuísaGlutamate receptors of the [alpha]-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type play an important role in synaptic plasticity and contribute to cell death under excitotoxic conditions. AMPA receptors form heterotetramers of four homologous subunits (GluR1-4), which exist in two functionally different isoforms, flip and flop, generated by alternative splicing. We identified transcripts for alte...
Differential modulation of CXCR4 and CD40 protein levels by skin sensitizers an...
Neves, Bruno Miguel; Cruz, Maria Teresa; Francisco, Vera; Gonçalo, Margarida; Figueiredo, Américo; Duarte, Carlos B.; Lopes, Maria CelesteThe development of non-animal methods for skin sensitization testing is an urgent challenge. Some of the most promising in vitro approaches are based on the analysis of phenotypical and functional modifications induced by sensitizers in dendritic cell models. In this work, we evaluated, for the first time, a fetal skin-derived dendritic cell line (FSDC) as a model to discriminate between sensitizers and irritan...
Validation of internal control genes for expression studies: Effects of the neu...
Santos, Ana Rita A.; Duarte, Carlos B.The stability of expression of an internal control is required for accurate and reliable normalization in quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) experiments. However, expression of commonly used reference genes can be regulated under specific experimental conditions, particularly in response to stimuli that exert multiple effects on gene expression. The neurotrophin bra...
BDNF regulates the expression and traffic of NMDA receptors in cultured hippoca...
Caldeira, Margarida V.; Melo, Carlos V.; Pereira, Daniela B.; Carvalho, Ricardo F.; Carvalho, Ana Luísa; Duarte, Carlos B.The neurotrophin BDNF regulates the activity-dependent modifications of synaptic strength in the CNS. Physiological and biochemical evidences implicate the NMDA glutamate receptor as one of the targets for BDNF modulation. In the present study, we investigated the effect of BDNF on the expression and plasma membrane abundance of NMDA receptor subunits in cultured hippocampal neurons. Acute stimulation of hippoc...
PKC Anchoring to GluR4 AMPA Receptor Subunit Modulates PKC-Driven Receptor Phos...
Gomes, André R.; Correia, Susana S.; Esteban, José A.; Duarte, Carlos B.; Carvalho, Ana LuísaChanges in the synaptic content of 03B1-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)2013type glutamate receptors lead to synaptic efficacy modifications, involved in synaptic plasticity mechanisms believed to underlie learning and memory formation. Early in development, GluR4 is highly expressed in the hippocampus, and GluR4-containing AMPA receptors are inserted into synapses. During synapse maturatio...
Brain-derived neurotrophic factor regulates the expression and synaptic deliver...
Caldeira, Margarida V.; Melo, Carlos V.; Pereira, Daniela B.; Carvalho, Ricardo; Correia, Susana S.; Backos, Donald S.; Carvalho, Ana LuísaBrain-derived neurotrophic factor (BDNF) plays an important role in synaptic plasticity in the hippocampus, but the mechanisms involved are not fully understood. The neurotrophin couples synaptic activation to changes in gene expression underlying long term potentiation and short term plasticity. Here we show that BDNF acutely up-regulates GluR1, GluR2, and GluR3 alpha-amino-3-hydroxy-5-methyl-4-isoxazole propi...
The interaction between dopamine D2-like and beta-adrenergic receptors in the p...
Montezinho, Liliana P.; Castro, M. Margarida C. A.; Duarte, Carlos B.; Penschuck, Silke; Geraldes, Carlos F. G. C.; Mørk, ArneSeveral studies have suggested the involvement of biogenic monoaminergic neurotransmission in bipolar disorder and in the therapy for this disease. In this study, the effects of the mood-stabilizing drugs lithium, carbamazepine or valproate on the dopaminergic and adrenergic systems, particularly on D2-like and 03B2-adrenergic receptors, were studied both in cultured rat cortical neurones and in rat prefrontal ...
Sample sonication after trichloroacetic acid precipitation increases protein re...
Manadas, Bruno J.; Vougas, Konstantinos; Fountoulakis, Michael; Duarte, Carlos B.Protein precipitation with TCA followed by acetone washing is frequently used to clean samples before 2-DE. However, the difficulty in solubilizing TCA-precipitated proteins causes some variability in 2-D gels and makes it difficult to detect some proteins. In this work we show that sonication of the samples, after TCA precipitation followed by elution in sample buffer, increases total protein recovery, and imp...
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