Multiple bond 13C-13C spin-spin coupling provides complementary information in ...

• Author(s): Carvalho, Rui A. ; Babcock, Evelyn E. ; Jeffrey, F. Mark H. ; Sherry, A. Dean ; Malloy, Craig R.
• Date: 1999
• Persistent ID: http://hdl.handle.net/10316/8105
• Origin: Estudo Geral - Universidade de Coimbra
• Document Type: Article
• Language: English

Description

Most 13C nuclear magnetic resonance (NMR) isotopomer analyses relate a metabolic index of interest to populations of 13C isotopomers as reported by one-bond 13C-13C spin-spin couplings. Metabolic conditions that produce highly enriched citric acid cycle intermediates often lead to 13C NMR spectra of metabolites such as glutamate that show extra multiplets due to long-range couplings. It can be demonstrated from 13C NMR spectra of hearts perfused with mixtures of acetate plus propionate that multiplets in glutamate C2 arising from 3J25 coupling provide a direct readout of acetyl-CoA fractional enrichment (FC1 and FC3), while multiplets in glutamate C5 arising from 2J35 and 3J25 couplings quantitatively reflect enrichment of the anaplerotic substrate. Magn Reson Med 42:197-200, 1999. © 1999 Wiley-Liss, Inc. http://dx.doi.org/10.1002/(SICI)1522-2594(199907)42:1<197::AID-MRM26>3.0.CO;2-5