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A (13)C isotopomer kinetic analysis of cardiac metabolism: influence of altered...
Carvalho, Rui A.; Rodrigues, Tiago B.; Zhao, Piyu; Jeffrey, F. Mark H.; Malloy, Craig R.; Sherry, A. DeanRat hearts were perfused with mixtures of [3-(13)C]pyruvate and [3-(13)C]lactate (to alter cytosolic redox) at low (0.5 mM) or high (2.5 mM) Ca(2+) concentrations to alter contractility. Hearts were frozen at various times after exposure to these substrates, were extracted, and were then analyzed by (13)C NMR spectroscopy. The time-dependent multiplets observed in the (13)C NMR resonances of glutamate in all he...
Multiple bond 13C-13C spin-spin coupling provides complementary information in ...
Carvalho, Rui A.; Babcock, Evelyn E.; Jeffrey, F. Mark H.; Sherry, A. Dean; Malloy, Craig R.Most 13C nuclear magnetic resonance (NMR) isotopomer analyses relate a metabolic index of interest to populations of 13C isotopomers as reported by one-bond 13C-13C spin-spin couplings. Metabolic conditions that produce highly enriched citric acid cycle intermediates often lead to 13C NMR spectra of metabolites such as glutamate that show extra multiplets due to long-range couplings. It can be demonstrated from...
13C isotopomer analysis of glutamate by heteronuclear multiple quantum coherenc...
Carvalho, Rui A.; Jeffrey, F. Mark H.; Sherry, A. Dean; Malloy, Craig R.13C has become an important tracer isotope for studies of intermediary metabolism. Information about relative flux through pathways is encoded by the distribution of 13C isotopomers in an intermediate pool such as glutamate. This information is commonly decoded either by mass spectrometry or by measuring relative multiplet areas in a 13C NMR spectrum. We demonstrate here that groups of glutamate 13C isotopomers...
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