Electrochemical detection of in situ adriamycin oxidative damage to DNA

• Author(s): Oliveira-Brett, A. M. ; Vivan, M. ; Fernandes, I. R. ; Piedade, J. A. P.
• Date: 2002
• Persistent ID: http://hdl.handle.net/10316/5192
• Origin: Estudo Geral - Universidade de Coimbra
• Subject(s): Adriamycin; DNA-biosensor; Oxidative damage; 8-Oxoguanine; Voltammetry
• Document Type: Article
• Language: English

Description

Adriamycin intercalation and in situ interaction with double helix DNA was investigated using a voltammetric DNA-biosensor. Oxidation and reduction of adriamycin molecules intercalated in double helix DNA were investigated in order to understand the in vivo mechanism of action with this anti-neoplasic drug. The results showed that the interaction of adriamycin with DNA is potential-dependent causing contact between DNA guanine and adenine bases and the electrode surface such that their oxidation is easily detected. A mechanism for adriamycin reduction and oxidation in situ when intercalated in double helix DNA immobilised onto the glassy carbon electrode surface is presented and the formation of the mutagenic 8-oxoguanine explained. http://www.sciencedirect.com/science/article/B6THP-44PVS7R-M/1/1474d369a4b1d556b84d548d2df25439