Electrochemistry of nanoscale DNA surface films on carbon

• Author(s): Oliveira-Brett, A. M. ; Paquim, A. M. Chiorcea ; Diculescu, V. C. ; Piedade, J. A. P.
• Date: 2006
• Persistent ID: http://hdl.handle.net/10316/5066
• Origin: Estudo Geral - Universidade de Coimbra
• Subject(s): Electrochemical DNA-biosensor; Nanoscale; Nanobioelectrochemistry; Surface films; Quercetin; Adriamycin
• Document Type: Article
• Language: English

Description

A DNA electrochemical biosensor is an integrated receptor-transducer device. The most important step in the development and manufacture of a sensitive DNA-biosensor for the detection of DNA-drug interactions is the immobilization procedure of the nucleic acid probe on the transducer surface. Magnetic A/C Mode atomic force microscopy (MAC Mode AFM) images in air were used to characterize two different procedures for immobilising nanoscale double-stranded DNA (dsDNA) surface films on carbon electrodes. Thin film dsDNA layers presented holes in the dsDNA film that left parts of the electrode surface uncovered while thicker films showed a uniform and complete coverage of the electrode. These two procedures for preparing dsDNA-biosensors were used to study the influence of reactive oxygen species (ROS) in the mechanism of DNA damage by quercetin, a flavonoid, and adriamycin, an anthracycline anticancer drug. The study of quercetin-DNA interactions in the presence of Cu(II) ions indicated that the formation of a quercetin-Cu(II) complex leads to the formation of ROS necessary to react with DNA, disrupting the helix and causing the formation of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo). Reduced adriamycin radicals are able to directly cause oxidative damage to DNA, generating 8-oxodGuo and ROS are not directly involved in this genomic mutagenic lesion. http://www.sciencedirect.com/science/article/B6T9K-4K8SCC9-3/1/dbc76b220aa06fdac69f6d05d4164192