Pertussis toxin prevents presynaptic inhibition by kainate receptors of rat hip...

• Author(s): A. Cunha, Rodrigo ; Malva, João O. ; Ribeiro, J. A.
• Date: 2000
• Persistent ID: http://hdl.handle.net/10316/4841
• Origin: Estudo Geral - Universidade de Coimbra
• Subject(s): Kainate receptor; G protein; Protein kinase C; Hippocampus
• Document Type: Article
• Language: English

Description

Kainate receptors are ionotropic receptors, also reported to couple to Gi/Go proteins, increasing neuronal excitability through disinhibition of neuronal circuits. We directly tested in hippocampal synaptosomes if kainate receptor-mediated inhibition of GABA release involved a metabotropic action. The kainate analogue, domoate (3 [mu]M), inhibited by 24% [3H]GABA-evoked release, an effect reduced by 76% in synaptosomes pre-treated with pertussis toxin. Protein kinase C inhibition attenuated by 82% domoate-induced inhibition of GABA release whereas protein kinase C activation did not change kainate receptor binding. Thus, domoate inhibition of GABA release recruits Gi/Go proteins and a protein kinase C pathway. http://www.sciencedirect.com/science/article/B6T36-3YRVBPH-6/1/a4d0cd3bf73cd3eea2d7e666afc0bdd4