Author(s):
Candeias, M 
; Alves-Pereira, I ; Ferreira, Rui
Date: 2012
Persistent ID: http://hdl.handle.net/10174/7267
Origin: Repositório Científico da Universidade de Évora
Subject(s): cell viability; DPPH
Document details
Glutathione reductase play a key role in the differential response of Saccharom...
Description
The isoproturon is a systemic herbicide applied in pre- and post-emergence control of annual grasses and broad-leaved weeds in winter crops that block photosynthesis, being listed by European Union as special substance that threaten the earth surface. Probably, can generate reactive oxygen species blocking the respiratory chain and cause oxidative stress and cell death. So, the main purpose of this study was to evaluate the effect of isoproturon on the antioxidant capacity of the wild-type Saccharomyces cerevisiae UE-ME3 and IGC-4072 strains. The UE-ME3 strain showed an adaptive response to this phenylurea, showing a growth similar to that control cells, an increase of non-protein thiols content, cell viability and glutathione antioxidant capacity, response that depend on glutathione reductase and glucose-6-phosphate dehydrogenase activities. However, the IGC-4072 strain in the presence of isoproturon showed cell death, exhibiting a poorly growth, dry weight and cell viability decrease, as well as a significant increase of free radicals scavenger (DPPH) and glutathione peroxidase activity.
The isoproturon is a systemic herbicide applied in pre- and post-emergence control of annual grasses and broad-leaved weeds in winter crops that block photosynthesis, being listed by European Union as special substance that threaten the earth surface. Probably, can generate reactive oxygen species blocking the respiratory chain and cause oxidative stress and cell death. So, the main purpose of this study was to evaluate the effect of isoproturon on the antioxidant capacity of the wild-type Saccharomyces cerevisiae UE-ME3 and IGC-4072 strains. The UE-ME3 strain showed an adaptive response to this phenylurea, showing a growth similar to that control cells, an increase of non-protein thiols content, cell viability and glutathione antioxidant capacity, response that depend on glutathione reductase and glucose-6-phosphate dehydrogenase activities. However, the IGC-4072 strain in the presence of isoproturon showed cell death, exhibiting a poorly growth, dry weight and cell viability decrease, as well as a significant increase of free radicals scavenger (DPPH) and glutathione peroxidase activity.
Document Type
Part of book or chapter of book
Language English
Editor(s) Mendez-Vilas, A
Language English
Editor(s) Mendez-Vilas, A
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