Author(s):
Santos-Silva, Celeste 
; Louro, Rogério ; Ragonezi, Carla ; Caldeira, Ana Teresa ; Martins, Rosário ; Klimaszewska, Kristina ; Dias, Luis ; Ganhao, Elsa ; Miralto, Otília ; Zavattieri, Amely
Date: 2011
Persistent ID: http://hdl.handle.net/10174/4370
Origin: Repositório Científico da Universidade de Évora
Subject(s): acclimation; Pinus pinea; Pisolithus arhizus
Description
The ICAAM Institute aims to study Mediterranean forest ecosystems in all
aspects. Our Plant Breeding and Biotechnology Laboratory of ICAAM has
always been involved in biotechnology of Mediterranean woody species,
and has developed various in vitro techniques for vegetative propagation of
Quercus and Pinus (Ragonezi et al. 2010, Zavattieri et al. 2009). The power
of clonal propagation for the improvement of these woody species is
indisputable. However, despite the fact that a lot of improvement in
micropropagation has been made, we have always faced problems in the
rooting phase (lack or reduced root growth), acclimation (water stress, loss
of plants) and transfer to the field (low adaptability, low plant
establishment).
In this context, a few years ago we found an adequate “natural solution” for
the in vitro mycorrhization. Different ECM fungi from pure and mixed
stands of Pinus pinea were tested for their ability to enhance root formation
and root sustainability, acclimation performance and survival of plants.
Results of growth and physiological parameters during the in vitro and ex
vitro acclimation phases of microplants inoculated with Phisolithus arhizus growing in different substrates and different conditions during ex vitro
development and colonization will be presented.
Literature
Ragonezi, C., Castro, M.R., Klimaszewska, K., Lima, M., Zavattieri, M.A.
2010: Influence of light quality and intensity on adventitious root
formation in microshoots of Pinus pinea L. Acta Hort., (ISHS) 865:287-
291.
Zavattieri, A., Lima, M., Sobral, V., Oliveira, P., Costa, A. 2009: Effects
of carbon source carbon concentration and culture conditions on in vitro
rooting of Pinus pinea L. microshoots. Acta Hort., (ISHS) 812:173-180.
