Stone pine (Pinus pinea L.), like other conifers, forms ectomycorrhizas (ECM), which have beneficial impact on plant growth in natural environments and forest ecosystems. An in vitro co-culture of stone pine microshoots with pure mycelia of isolated ECM sporocarps was used to overcome the root growth cessation not only in vitro but also to improve root development during acclimation phase. Pisolithus arhizus (S...
Oral presentation Micropropagation of recalcitrant pine (Pinus pinea L.). An overview of the effects of ectomycorrhizal inoculation Carla Ragonezi1; Ana Teresa Caldeira2 3; Maria do Rosário Martins2,5; Otília Miralto5,6; Luís Silva Dias6; Elsa Ganhão6, Krystyna Klimaszewska4, Amely Zavattieri5,6 1 Plant Breeding and Biotechnology Laboratory, University of Évora, Ap. 94, 7002-554 Évora, Portugal 2 Department o...
LOCUS JQ066791 439 bp DNA linear PLN 20-MAR-2012 DEFINITION Lactarius deliciosus isolate UEZB1 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence. ACCESSION JQ066791 VERSION JQ066791.1 GI:380467948 KEYWORDS . SOURCE...
http://www.ncbi.nlm.nih.gov/nuccore/HQ896485.1.
The ICAAM Institute aims to study Mediterranean forest ecosystems in all aspects. Our Plant Breeding and Biotechnology Laboratory of ICAAM has always been involved in biotechnology of Mediterranean woody species, and has developed various in vitro techniques for vegetative propagation of Quercus and Pinus (Ragonezi et al. 2010, Zavattieri et al. 2009). The power of clonal propagation for the improvement of thes...
Mesmo quando estimulada pela aplicação exógena de reguladores de crescimento, a capacidade de enraizamento da oliveira, por estacaria semi-lenhosa, encontra-se muito dependente do genótipo utilizado. É assim possível encontrar cultivares como a ‘Cobrançosa’, onde se atingem sem dificuldade taxas de formação de raízes adventícias próximas dos 70%, e cultivares como a “Galega vulgar”, onde só muito esporadicament...
Stone pine (Pinus pinea L.) is an economically important forest tree in Mediterranean climates and has been the target for selection efforts through micropropagation. Previous attempts on microshoots, derived from mature seed cotyledons, reached incipient rooting after induction with a combination of auxin and hypertonic shock, but their development in vitro was not sustained. At this stage, co-culturing pla...
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