Author(s):
Oliveira, Ivo 
; Sousa, A. ; Valentão, P. ; Andrade, P.B. ; Ferreira, Isabel C.F.R. ; Ferreres, F. ; Bento, Albino ; Seabra, R.M. ; Estevinho, Leticia M. ; Pereira, J.A.
Date: 2007
Persistent ID: http://hdl.handle.net/10198/753
Origin: Biblioteca Digital do IPB
Subject(s): Hazel leaves; Phenolics; Antioxidant potential; Antimicrobial activity
Description
Aqueous extracts of leaves of different hazel (Corylus avellana L.) cultivars (Cv. M. Bollwiller, Fertille de Coutard and Daviana), were
analysed by reversed-phase HPLC/DAD for the definition of their phenolic composition. Antioxidant potential was assessed by the
reducing power assay, and the scavenging effect on DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals and b-carotene linoleate model system.
Their antimicrobial capacity was also tested against Gram positive (Bacillus cereus, Bacillus subtilis, Staphylococcus aureus) and
Gram negative bacteria (Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae) and fungi (Candida albicans, Cryptococcus neoformans).
Eight phenolic compounds were identified: 3-, 4- and 5-caffeoylquinic acids, caffeoyltartaric acid, p-coumaroyltartaric acid,
myricetin-rhamnoside, quercetin 3-rhamnoside and kaempferol 3-rhamnoside. A p-coumaric acid, three myricetin and one quercetin
derivatives were also detected. The hazel leaves extract presented high antioxidant activity in a concentration-dependent way, in general
with similar behaviour of all cultivars. Gram positive bacteria revealed to be very sensitive to hazel leaf extract (MIC 0.1 mg/ml for
B. cereus and S. aureus and 1 mg/ml for B. subtilis). However, Gram negative and the fungi displayed much lower sensitivity, being
P. aeruginosa and C. albicans resistant at 100 mg/ml. Cv. M. Bollwiller exhibited the most potent antimicrobial activity.
