Rapid HPLC method for simultaneous detection of aflatoxins and cyclopiazonic fr...

• Autor(es): Soares, Célia ; Rodrigues, Paula ; Freitas-Silva, Otniel ; Abrunhosa, Luís ; Venâncio, Armando
• Data: 2010
• Identificador Persistente: http://hdl.handle.net/10198/4240
• Origem: Biblioteca Digital do IPB
• Assunto(s): Multimycotoxin detection; Filamentous fungi
• Tipo de Documento: Documento de conferência
• Idioma: Inglês

Descrição

Mycotoxins are secondary metabolites produced by moulds and are an important world-wide food safety concern. Among the most relevant mycotoxigenic producer fungi are some Aspergillus species in particular those belonging to the Aspergillus section Flavi. These are known to produce the highly carcinogenic aflatoxins in agricultural commodities. Due to its impact in animal and human health, these species are among the most intenSively studied ones, being well known producers of aflatoxins (AFB1 , AFB2; AFG1 and AFG2) and cyciopiazonic acid (CPA). Aflatoxins are mainly produced by some strains of Aspergillus flavus and Aspergillus nomius and by most, if not all, strains of Aspergillus parasiticus. On the other hand, cyclopiazonic acid, which naturally occurs in a large variety of crop products as a co-contaminant with aflatoxins, is mainly produced by Aspergillus flavus strains. Together they have been shown to cause health problems in animals and humans, resulting in important economic losses. The production of CPA by Aspergillus section Flavi may also be routinely used for identification purposes since A. parasiticus, A. flavus and A. nomius, exhibit different mycotoxin profiles. The detection and quantification of both these mycotoxins is usually done separately by HPLC with UV detection for CPA and fluorescence detection after post-column derivatization for aflatoxins. There isn't a chromatographic method available to detect simultaneously CPA and the main four aflatoxins.