Liquid-liquid extraction in the presence of electrolytes of nisin and green flu...

• Autor(es): Mazzola, Priscila Gava ; Jozala, A. F. ; Magalhães, Pérola de Oliveira ; Pessoa Júnior, Adalberto ; Penna, T. C. V.
• Data: 2008
• Identificador Persistente: http://hdl.handle.net/1822/9235
• Origem: RepositóriUM - Universidade do Minho
• Assunto(s): Liquid-liquid extraction; Green fluorescent protein; Nisin
• Tipo de Documento: Documento de conferência
• Idioma: Inglês

Descrição

In the biotechnology field, it has been suggested that extractions in two-phase aqueous complex-fluid systems can possibly be used instead of, or as complementary processes to, the more typical chromatographic operations, to reduce the cost of the downstream processing of many biological products (Lam et al., 2004; Mazzola et al., 2006). This method offer attractive conditions to be applied in this study, thereby two-phase systems can be exploited in separation science for the extraction/purification of desired biomolecules directly on the culture medium (Mazzola et al., 2008). This study aimed to evaluate the aqueous two phase system (ATPS) composed by a nonionic surfactant, Triton X-114 (TX), in presence or absence of electrolytes, to separate two interesting biomolecules: nisin and recombinant green fluorescent protein (GFP). Results indicated that nisin partitions preferentially to the micelle rich-phase, with significant antimicrobial activity increase (up to 10-fold). GFP partitioned evenly between the phases in TX system without electrolytes.