Encontrados 8 documentos, a visualizar página 1 de 1
Extraction of fibrinolytic proteases from Streptomyces sp. DPUA1576 using PEG-p...
Silva, Germana Michelle de Medeiros e; Marques, Daniela Araújo Viana; Porto, Tatiana Souza; Lima Filho, José L.; Teixeira, J. A.Extraction of fibrinolytic protease from Streptomyces sp. DPUA1576 fermentation broth was performed using polyethylene glycol (PEG) – phosphate salts aqueous two phase system (ATPS). The influence of pH, PEG molar mass, PEG and phosphate concentrations on fibrinolytic protease partition coefficient (K) were evaluated by 24 full factorial design. Fibrinolytic protease partitioned preferentially to the (PEG)-rich...
A stable liquid–liquid extraction system for clavulanic acid using polymer-base...
Pereira, Jorge F. B.; Santos, Valéria Carvalho; Johansson, Hans-Olof; Teixeira, J. A.; Pessoa Junior, AdalbertoThe partitioning of Clavulanic Acid (CA) in a novel inexpensive and stable aqueous two-phase system (ATPS) composed by poly(ethylene glycol) (PEG) and sodium polyacrylate (NaPA) has been studied. The aqueous two-phase systems are formed by mixing both polymers with a salt (NaCl or Na2SO4) and an aqueous solution of CA. The stability of CA on the presence of both polymers was investigated and it was observed tha...
Aqueous two-phase micellar systems in an oscillatory flow micro-reactor: Study ...
Lopes, A. M.; Silva, D. P.; Vicente, A. A.; Pessoa Júnior, Adalberto; Teixeira, J. A.Aqueous two-phase micellar systems (ATPMS) are micellar surfactant solutions with physical properties that make them very efficient for the extraction/concentration of biological products. In this work the main proposal that has been discussed is the possible applicability and importance of a novel oscillatory flow micro-reactor (micro-OFR) envisaged for parallel screening and/or development of industrial biopr...
Processing of byproducts to improve nisin production by Lactococcus lactis
Jozala, A. F.; Silva, D. P.; Vicente, A. A.; Teixeira, J. A.; Pessoa Júnior, Adalberto; Penna, Thereza C. V.In the last years, disposal from dairy industries have received a special attention due its polluting power in the environment. For this reason, studies have obtained a positive support to develop different alternatives to recycle milk whey components. One of them is its utilization as culture media, aiming to produce biomolecules with noble applications. Nisin is an extracellular peptide, produced by Lacto...
Stability of clavulanic acid under variable pH, ionic strength and temperature ...
Santos, Valéria Carvalho; Pereira, Jorge F. Brandão; Haga, Raquel Brandão; Rangel-Yagui, Carlota O.; Teixeira, J. A.; Converti, AttilioClavulanic acid (CA) is a β-lactam antibiotic that alone exhibits only weak antibacterial activity, but is a potent inhibitor of β-lactamases enzymes. For this reason it is used as a therapeutic in conjunction with penicillins and cephalosporins. However, it is a well-known fact that it is unstable not only during its production phase, but also during downstream processing. Therefore, the main objective of this...
Liquid-liquid extraction in the presence of electrolytes of nisin and green flu...
Mazzola, Priscila Gava; Jozala, A. F.; Magalhães, Pérola de Oliveira; Pessoa Júnior, Adalberto; Penna, T. C. V.In the biotechnology field, it has been suggested that extractions in two-phase aqueous complex-fluid systems can possibly be used instead of, or as complementary processes to, the more typical chromatographic operations, to reduce the cost of the downstream processing of many biological products (Lam et al., 2004; Mazzola et al., 2006). This method offer attractive conditions to be applied in this study, there...
Nisin biotechnological production : evaluation and perspectives in the developm...
Jozala, A. F.; Silva, D. P.; Vicente, A. A.; Pessoa Júnior, Adalberto; Penna, T. C. V.; Teixeira, J. A.Nisin is a commercially available bacteriocin of the lantibiotic group produced by Lactococcus lactis ATCC 11454. The importance of nisin as an effective preservative is the fact that its spectrum of inhibitory activity includes almost all Gram-positive, Gram-negative and spoilage bacteria. With several favorable characteristics, this compound has been widely used as a natural food biopreservative. On the other...
Partial purification of glucose-6-phosphate dehydrogenase by aqueous two-phase ...
Ribeiro, Marcela Zanella; Silva, Daniel Pereira da; Vitolo, Michele; Roberto, Inês Conceição; Pessoa Júnior, AdalbertoGlucose-6-phosphate dehydrogenase (G6PDH) is an important enzyme used in biochemical and medical studies and in several analytical methods that have industrial and commercial application. This work evaluated the extraction of G6PDH in aqueous two-phase system (ATPS) of poly(ethyleneglycol) (PEG)/phosphate buffer, using as enzyme source a medium prepared through commercial baker’s yeast disruption. Firstly, the ...
| Financiadores do RCAAP | |||||||
|
|
|
|
|
|
||