Document details

Application of the Cre-loxP system for multiple gene disruption in the yeast kl...

Author(s): Ribeiro, Orquídea cv logo 1 ; Gombert, Andreas Karoly cv logo 2 ; Teixeira, J. A. cv logo 3 ; Domingues, Lucília cv logo 4

Date: 2007

Persistent ID: http://hdl.handle.net/1822/6738

Origin: RepositóriUM - Universidade do Minho

Subject(s): Cre-loxP system; Kluyveromyces marxianus; Gene disruption; β-Galactosidase; Gene targeting efficiency


Description
The yeast Kluyveromyces marxianus presents several interesting features that make this species a promising industrial yeast for the production of several compounds. In order to take full advantage of this yeast and its particular properties, proper tools for gene disruption and metabolic engineering are needed. The Cre-loxP system is a very versatile tool that allows for gene marker rescue, resulting in mutant strains free of exogenous selective markers, which is a very important aspect for industrial application. As the Cre-loxP system works in some non-conventional yeasts, namely Kluyveromyces lactis, we wished to know whether it also works in K. marxianus. Here, we report the validation of this system in K. marxianus CBS 6556, by disrupting two copies of the LAC4 gene, which encodes a β-galactosidase activity.
Document Type Article
Language English
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