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Diferenças no Perfil Microbiológico entre as Infecções da Comunidade, Associada...

Introdução: Analisamos e comparamos os perfis microbiológicos, das infecções intra-abdominais, urinárias, respiratórias e bacteriemias, conforme o local de aquisição: da comunidade, com separação das infecções associadas a cuidados de saúde, ou nosocomiais.Material e Métodos: Coorte prospectiva, desenvolvida num hospital universitário ao longo de um ano. Critério de inclusão: cumprir a definição de infecção int...

Random and direct mutagenesis to enhance protein secretion in Ashbya gossypii

To improve the general secretion ability of the biotechnologically relevant fungus Ashbya gossypii, random mutagenesis with ethyl methane sulfonate (EMS) was performed. The selection and screening strategy followed revealed mutants with improved secretion of heterologous Trichoderma reesei endoglucanase I (EGI), native α-amylase and/or native β-glucosidase. One mutant, S436, presented 1.4- to 2-fold increases i...

Burnout in intensive care units - a consideration of the possible prevalence an...

Background: The provision of Intensive Care (IC) can lead to a health care provider’s physical, psychological and emotional exhaustion, which may develop into burnout. We notice the absence of specific studies regarding this syndrome in Portuguese Intensive Care Units (ICUs). Our main objective is to study the incidence and risk factors of burnout in Portuguese ICUs. Methods: A self-fulfilment questionnaire con...

Physiological characterization of Ashbya gossypii and strain development for re...

Tese de doutoramento em Engenharia Quimica e Biológica ; Ashbya gossypii is a phytopathogenic hemiascomycete belonging to the Saccharomycetaceae family. This fungus has attracted attention because of its natural ability to produce riboflavin (vitamin B2) as a detoxifying and protective mechanism being used at an industrial level as a biotechnological important producer of riboflavin. The genome sequence of thi...

Nutritional requirements and strain heterogeneity in Ashbya gossypii

Colony radial growth rates and specific growth rates of three related Ashbya gossypii strains ATCC10895, IMI31268, MUCL29450 and an unrelated strain, CBS109.26, were measured on various carbon and nitrogen sources at pH 4.5 and pH 6.5 to elucidate physiological growth requirements and strain differences. All strains grew on yeast extract or ammonium as nitrogen sources, but not on nitrate. Substantial growth at...

Expression of Trichoderma reesei cellulases CBHI and EGI in Ashbya gossypii

To explore the potential of Ashbya gossypii as a host for the expression of recombinant proteins and to assess whether protein secretion would be more similar to the closely related Saccharomyces cerevisiae or to other filamentous fungi, endoglucanase I (EGI) and cellobiohydrolase I (CBHI) from the fungus Trichoderma reesei were successfully expressed in A. gossypii from plasmids containing the two micron seque...

Biotechnological versatility of riboflavin producer Ashbya gossypii

Este resumo faz parte de: Book of abstracts of the Meeting of the Institute for Biotechnology and Bioengineering, 2, Braga, Portugal, 2010. A versão completa do livro de atas está disponível em: http://hdl.handle.net/1822/10968

Expression of Trichoderma reesei cellulases in the fungus Ashbya gossypii

Multiple gene disruption and marker rescue in the yeast Kluyveromyces marxianus

Application of the Cre-loxP system for multiple gene disruption in the yeast kl...

The yeast Kluyveromyces marxianus presents several interesting features that make this species a promising industrial yeast for the production of several compounds. In order to take full advantage of this yeast and its particular properties, proper tools for gene disruption and metabolic engineering are needed. The Cre-loxP system is a very versatile tool that allows for gene marker rescue, resulting in mutant ...