Author(s):
Oliveira, Rosário 
; Gomes, F. I. ; Ceri, H. ; Teixeira, P. ; Cerca, Nuno
Date: 2010
Persistent ID: http://hdl.handle.net/1822/26049
Origin: RepositóriUM - Universidade do Minho
Document details
Effect of single versus antibiotic combinations on Staphylococcus epidermidis b...
Description
In this study five clinical isolates strains were used, and nine antibiotics at breakpoint concentrations: vancomycin, tetracycline, rifampicin, gentamicin, cefazolin, cephalothin, levofloxacine, daptomycin and clindamycin were tested. 48 hours biofilms were grown on Calgary Biofilm Device (CBD) and challenged overnight with antibiotics alone and in combination. Biofilm cells viability was determined by colony forming units (cfu). Afterwards, the effect of the most active antibiotics combinations against S. epidermidis biofilm on genetic expression of some genes of interest such as: icaA, icaR, sarA and rsbU was determined by real-time PCR. Although biofilms were generally insensitive to individual antibiotics, they were more susceptible to combinations. Levofloxacine was a constituent of almost all the combinations active against S. epidermidis biofilm pointing to be part of any antibiotic therapy directed against biofilms of these organisms.
In this study five clinical isolates strains were used, and nine antibiotics at breakpoint concentrations: vancomycin, tetracycline, rifampicin, gentamicin, cefazolin, cephalothin, levofloxacine, daptomycin and clindamycin were tested. 48 hours biofilms were grown on Calgary Biofilm Device (CBD) and challenged overnight with antibiotics alone and in combination. Biofilm cells viability was determined by colony forming units (cfu). Afterwards, the effect of the most active antibiotics combinations against S. epidermidis biofilm on genetic expression of some genes of interest such as: icaA, icaR, sarA and rsbU was determined by real-time PCR. Although biofilms were generally insensitive to individual antibiotics, they were more susceptible to combinations. Levofloxacine was a constituent of almost all the combinations active against S. epidermidis biofilm pointing to be part of any antibiotic therapy directed against biofilms of these organisms.
Document Type
Conference Object
Language English
Language English
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