Plasmid-mediate transfer of FLO-1 into industrial Saccharomyces cerevisiae PE-2...

• Author(s): Gomes, Daniel G. ; Guimarães, Pedro M. R. ; Pereira, Francisco B. ; Teixeira, J. A. ; Domingues, Lucília
• Date: 2012
• Persistent ID: http://hdl.handle.net/1822/22505
• Origin: RepositóriUM - Universidade do Minho
• Subject(s): Yeast flocculation; VHG bio-ethanol production; Repeat-batch operation; Industrial Saccharomyces cerevisiae strains
• Document Type: Article
• Language: English

Description

The flocculation gene FLO1 was transferred into the robust industrial strain Saccharomyces cerevisiae PE-2 by the lithium acetate method. The recombinant strain showed a fermentation performance similar to that of the parental strain. In 10 repeat-batch cultivations in VHG medium with 345 g glucose/L and cell recycling by flocculation–sedimentation, an average final ethanol concentration of 142 g/L and an ethanol productivity of 2.86 g/L/h were achieved. Due to the flocculent nature of the recombinant strain it is possible to reduce the ethanol production cost because of lower centrifugation and distillation costs.