Author(s):
Carneiro, S. 
; Villas-Bôas, S. G. ; Rocha, I. ; Ferreira, E. C.
Date: 2012
Persistent ID: http://hdl.handle.net/1822/22168
Origin: RepositóriUM - Universidade do Minho
Document details
Applying a metabolic footprinting approach to characterize the impact of the re...
Description
In this study metabolic footprinting was applied to evaluate the metabolic consequences of protein overproduction at slow growth conditions (μ = 0.1 h-1). The extracellular metabolites detected by gas chromatography-mass spectrometry characterized the metabolic footprints before and after the induction of the recombinant protein production (i.e. pre- and post-induction phases). Metabolic footprinting enabled the discrimination between the two growth phases and ex-posed significant metabolic alterations in the extracellular milieu during the re-combinant processes.
In this study metabolic footprinting was applied to evaluate the metabolic consequences of protein overproduction at slow growth conditions (μ = 0.1 h-1). The extracellular metabolites detected by gas chromatography-mass spectrometry characterized the metabolic footprints before and after the induction of the recombinant protein production (i.e. pre- and post-induction phases). Metabolic footprinting enabled the discrimination between the two growth phases and ex-posed significant metabolic alterations in the extracellular milieu during the re-combinant processes.
Document Type
Article
Language English
Language English
| Financiadores do RCAAP | |||||||
|
|
|
|
|
|
||