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Metabolic responses to recombinant bioprocesses in Escherichia coli

Escherichia coli has been widely used for the production of recombinant proteins. However, the unbalances between host metabolism and recombinant biosynthesis continue to hamper the efficiency of these recombinant bioprocesses. The additional drainage of biosynthetic precursors toward recombinant processes burdens severely the metabolism of cells that, ultimately, elicits a series of stress responses, reducing ...

Influence of the RelA activity on E. coli metabolism by metabolite profiling of...

Metabolite profiling of E. coli W3110 and the isogenic ∆relA mutant cells was used to characterize the RelA-dependent stringent control of metabolism under different growth conditions. Metabolic profiles were obtained by gas chromatography–mass spectrometry (GC-MS) analysis and revealed significant differences between E. coli strains grown at different conditions. Major differences between the two strains were ...

Applying a metabolic footprinting approach to characterize the impact of the re...

In this study metabolic footprinting was applied to evaluate the metabolic consequences of protein overproduction at slow growth conditions (μ = 0.1 h-1). The extracellular metabolites detected by gas chromatography-mass spectrometry characterized the metabolic footprints before and after the induction of the recombinant protein production (i.e. pre- and post-induction phases). Metabolic footprinting enabled th...

Exploring current knowledge on Escherichia coli stringent response via text mining

Investigating metabolic responses in recombinant E. coli cultures using metabol...

Metabolic footprint analysis of recombinant Escherichia coli strains during fed...

Metabolic footprinting has become a valuable analytical approach for the characterization of phenotypes and the distinction of specific metabolic states resulting from environmental and/or genetic alterations. The metabolic impact of heterologous protein production in Escherichia coli cells is of particular interest, since there are numerous cellular stresses triggered during this process that limit the overall...

Semantic annotation of biological concepts interplaying microbial cellular resp...

Background Automated extraction systems have become a time saving necessity in Systems Biology. Considerable human effort is needed to model, analyse and simulate biological networks. Thus, one of the challenges posed to Biomedical Text Mining tools is that of learning to recognise a wide variety of biological concepts with different functional roles to assist in these processes. Results Here, we present ...

Metabolomics as a tool to understand E. coli metabolic responses in recombinant...

Challenges in integrating E. coli molecular biology data

One key challenge in Systems Biology is to provide mechanisms to collect and integrate the necessary data to be able to meet multiple analysis requirements. Typically, biological contents are scattered over multiple data sources and there is no easy way of comparing heterogeneous data contents. This work discusses ongoing standardisation and interoperability efforts and exposes integration challenges for the mo...

A study of the short and long-term regulation of E. coli metabolic pathways

The present study addresses the regulatory network of Escherichia coli and offers a global view of the short- and long-term regulation of its metabolic pathways. The regulatory mechanisms responsible for key metabolic activities and the structure behind such mechanisms are detailed. Most metabolic functions are dependent on the activity of transcriptional regulators over gene expression - the so-called long-ter...