Obtention of plant peroxidase and its potential for the decolorization of the r...

• Author(s): Silva, Maria Cristina ; Torres, Juliana A. ; Corrêa, Angelita D. ; Bernardes, Allana Maria ; Amorim, M. T. Pessoa de ; Santos, Custódio Donizete
• Date: 2012
• Persistent ID: http://hdl.handle.net/1822/21916
• Origin: RepositóriUM - Universidade do Minho
• Subject(s): Decolorization; Environmental biocatalysis; Peroxidase; Textile dyes; Turnip
• Document Type: Article
• Language: English

Description

Peroxidases can be used in the decolorization process. There is a growing interest for new sources of this enzyme and for obtaining economically viable processes. In this work, a low-cost vegetable peroxidase extraction process is proposed; the resulting enzyme is characterized to determine its optimum pH, temperature, and stability conditions, and it is then applied in the decolorization of reactive dye Remazol Turquoise G 133%. The turnip peroxidase (TP) was utilized as an enzymatic source. This enzyme exhibited maximum activity at pH 7.0, and it was active in the temperature range of 30 to 50 °C, which favors its use in industrial processes. Acetone was the most efficient solvent to induce precipitation. The removal of Remazol Turquoise G 133% was 56.0% complete after 50 min, while 41.0% of the same dye was removed with the commercial horseradish peroxidase enzyme in 50 min. TP presents potential as a viable alternative in the decolorization of textile wastewaters.