Immuno-Golgi as a tool for analyzing neuronal 3D-dendritic structure in phenoty...

• Author(s): Pinto, Luísa ; Pinheiro, António Mateus ; Morais, Mónica ; Bessa, J. M.
• Date: 2012
• Persistent ID: http://hdl.handle.net/1822/20448
• Origin: RepositóriUM - Universidade do Minho
• Subject(s): Golgi-cox; Immunofluorescence; Depression; Stress; Neuroplasticity; Neurogenesis
• Document Type: Article
• Language: Portuguese

Description

Characterization of neuronal dendritic structure in combination with the determination of specific neuronal phenotype or temporal generation is a challenging task. Here we present a novel method that combines bromodioxyuridine (BrdU) immunohistochemistry with Golgi-impregnation technique; with this simple noninvasive method, we are able to determine the tridimensional structure of dendritic arborization and spine shape of neurons born at a specific time in the hippocampus of adult animals. This analysis is relevant in physiological and pathological conditions in which altered neurogenesis is implicated, such as aging or emotional disorders