Fractionation and recovery of whey proteins by hydrophobic interaction chromato...

• Author(s): Santos, Maria João ; Teixeira, J. A. ; Rodrigues, L. R.
• Date: 2011
• Persistent ID: http://hdl.handle.net/1822/11930
• Origin: RepositóriUM - Universidade do Minho
• Subject(s): Hydrophobic interaction chromatography; whey proteins; b-lactoglobulin; recovery & isolation
• Document Type: Article
• Language: English

Description

A method for the recovery and fractionation of whey proteins from a whey protein concentrate (80%, w/w) by hydrophobic interaction chromatography is proposed. Standard proteins and WPC 80 dissolved in phosphate buffer with ammonium sulfate 1M were loaded in a HiPrep Octyl Sepharose FF column coupled to a fast protein liquid chromatography (FPLC) system and eluted by decreasing the ionic strength of the buffer using a salt gradient. The results showed that the most hydrophobic protein from whey is -lactalbumin and the less hydrophobic is lactoferrin. It was possible to recover 45.2% of -lactoglobulin using the HiPrep Octyl Sepharose FF column from the whey protein concentrate mixture with 99.6% purity on total protein basis