Author(s):
Martins, Joana 
; Cerqueira, M. A. ; Souza, B. W. S. ; Avides, Maria do Carmo ; Vicente, A. A.
Date: 2009
Persistent ID: http://hdl.handle.net/1822/10150
Origin: RepositóriUM - Universidade do Minho
Subject(s): Edible coating; Galactomannan; Nisin; Listeria monocytogenes; Ricotta cheese
Description
Antimicrobial packaging, besides protecting the product from the external environment, inhibits
or delays microorganism growth in foods and meets the actual demand of consumers for
healthier foods, containing less additives (Lopez‐Rubio, Gavara, & Lagaron, 2006).
Cheese is a ready‐to‐eat type of food that has been associated with foodborne listeriosis. Listeria
monocytogenes is an important ubiquitous foodborne pathogen which may contaminate foods at
pre‐ and post‐harvest stages of production. To overcome this problem bacteriocins could be
entrapped in a suitable edible coating applied to food. Nisin is an antimicrobial peptide produced
by Lactococcus lactis subsp. lactis. and research studies have revealed its ability to inhibit the
growth of some pathogenic bacteria (Sobrino‐López, & Martín‐Belloso, 2008).
The aim of this study was to evaluate the antimicrobial activity of coatings of galactomannans
from Gleditsia triacanthos incorporating nisin against L. monocytogenes during storage of Ricotta
cheese at 4 °C.
Three different treatments were tested: a control with no coating; a sample with coating
containing no nisin and a coating with 50 IU.g‐1 of nisin. Samples of cheese (20 g) were immersed
in 0.5 % w/v galactomannan solution containing glycerol (as plasticizer) (1.5 % v/v). To test the
effectiveness of the treatments Ricotta cheese samples were surface‐inoculated with a solution
containing approximately 1×106 CFU.ml‐1 of L. monocytogenes. Microbiological and physicalchemical
parameters (color change, pH, moisture content and weight loss) were monitored over
28 days for cheese stored at 4 ºC.
Among the three treatments, the combination of coating and nisin showed the best results,
followed by the coating containing no nisin. Counts of L. monocytogenes were lower (p<0.05) in
nisin‐containing coating that in no‐coated cheese. The nisin‐containing coating presented a
reduction from 5.1 to 4.4 log CFU.g‐1 after 2 days of storage. For samples coated with nisin,
reductions of 2.2 log CFU.g‐1 were achieved for samples after 7 days of storage.
These results suggest that the application of these coatings could be a potential food packaging
solution for the release of nisin in view of the control of L. monocytogenes spoilage in cheese.
