A link between nuclear RNA surveillance, the human exosome and RNA polymerase I...

• Autor(es): Almeida, Sérgio F. de ; García-Sacristán, Ana ; Custódio, Noélia ; Carmo-Fonseca, Maria
• Data: 2010
• Identificador Persistente: http://hdl.handle.net/10451/6360
• Origem: Repositório da Universidade de Lisboa
• Assunto(s): Eukaryotes; RNA, messenger; Exosome; RNA polymerase II
• Tipo de Documento: Artigo
• Idioma: Inglês

Descrição

©The Author(s) 2010. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/ by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. In eukaryotes, the production of mature messenger RNA that exits the nucleus to be translated into protein in the cytoplasm requires precise and extensive modification of the nascent transcript. Any failure that compromises the integrity of an mRNA may cause its retention in the nucleus and trigger its degradation. Multiple studies indicate that mRNAs with processing defects accumulate in nuclear foci or ‘dots’ located near the site of transcription, but how exactly are defective RNAs recognized and tethered is still unknown. Here, we present evidence suggesting that unprocessed β-globin transcripts render RNA polymerase II (Pol II) incompetent for termination and that this quality control process requires the integrity of the nuclear exosome. Our results show that unprocessed pre-mRNAs remain tethered to the DNA template in association with Pol II, in an Rrp6-dependent manner. This reveals an unprecedented link between nuclear RNA surveillance, the exosome and Pol II transcriptional termination.