Author(s):
Manaia, Céia M. 
; Nogales, Balbina ; Weiss, Norbert ; Nunes, Olga C.
Date: 2004
Persistent ID: http://hdl.handle.net/10400.14/6782
Origin: Veritati - Repositório Institucional da Universidade Católica Portuguesa
Description
A Gram-positive, molinate-degrading bacterium, strain ON4T (=DSM 13485T=LMG 21909T),
was isolated from a mixed bacterial culture able to mineralize the herbicide molinate. The strain was
strictly aerobic, oxidase- and catalase-positive and non-acid-fast, with a growth temperature of
10–41 6C. It contained the major menaquinone MK-9 and a cell-wall peptidoglycan based on
D-ornithine. 16S rDNA sequence analysis revealed that the strain formed a distinct line of descent
in the family Microbacteriaceae, showing the highest 16S rDNA similarity (~95 %) to members
of the genus Curtobacterium and ‘Brevibacterium helvolum’ DSM 20419 (=ATCC 13715).
The latter was reported to have the cell-wall peptidoglycan type B2c and the major menaquinone
MK-9, which are typical of Clavibacter, but it is clearly separated from this genus at the
phylogenetic level. Based on low values of 16S rDNA sequence similarity to previously
described genera and their distinctive phenotypic characteristics, it is proposed that strains
ON4T and ‘B. helvolum’ DSM 20419 be classified as two novel genera and species, with the
respective names Gulosibacter molinativorax gen. nov., sp. nov. and Pseudoclavibater helvolus
gen. nov., sp. nov.
