Histological assessment of small bowel hypoperfusion lesions in the pig

• Author(s): Oliveira, A. L. ; Ferreira, D. ; Vala, Helena
• Date: 2010
• Persistent ID: http://hdl.handle.net/10400.19/1584
• Origin: Repositório Científico do Instituto Politécnico de Viseu
• Subject(s): intestinal hypoperfusion; small intestine mucosa; pig; hypotension due to acute hemorrhage; quantitative morphological assessment; quantifying the degree of mucosal lost
• Document Type: Conference Object
• Language: English

Description

Background Authors propose the use of a quantitative morphological assessment for helping in studies concerning intestinal hypoperfusion. The method was applied to the small intestine mucosa stained with the standard hematoxylin and eosin, from pigs that underwent severe hypotension due to acute hemorrhage.,. Methods Six Large White pigs underwent total intravenous anesthesia with propofol and remifentanil. 25 ml/kg of arterial blood were passively removed from the femoral artery over 20 minutes. Volume was replaced using Ringer Lactate in Group 1 and Hydroxyethyl starch 130/0.4 in Group 2, with a delay of 20 minutes after the bleeding. One hour after the volume replacement, pigs were euthanized and the small intestine samples were taken for histopathological examination. Parameters were classified using two specific scales (Chiu, 1970; Çetin, 1995; Kaplan, 2007). Mucosal loss (ML) percentage and crypt:interstitium ratio were obtained (Faleiros, 2001). Results Inflammatory infiltrate was present in all animals, varying from grade 2 to grade 3. Hydropic cellular degeneration and epithelial detachment were more pronounced in duodenum and more noticeable in Group 1. In Group 1, ML percentage was 2.18±0.46 % in duodenum, 0.62±1,07% in jejunum and 0.45±0.77% in ileum. In Group 2 ML percentage was 0.75±1.30 in duodenum and 0±0% in the other intestinal segments. In the whole small intestine, was 1.08±0.78% in Group 1 and 0.25±0.35% in Group 2. Crypt:interstitium ratio did not presented significant differences between the groups. Conclusions The quantitative morphological assessment may be useful in quantifying the degree of mucosal lost in small intestine stained with hematoxylin and eosin, from pigs submitted to acute severe bleeding.