Fast growing fungi: a problem to be solved to achieve characterization of occup...

• Author(s): Nunes, Ana Carolina ; Domingues, A. ; Malta-Vacas, Joana ; Viegas, Carla
• Date: 2012
• Persistent ID: http://hdl.handle.net/10400.21/3842
• Origin: Repositório Científico do Instituto Politécnico de Lisboa
• Subject(s): Occupational health; Occupational exposure; Cork industry; Occupational asthma; Indoor air; Portugal
• Document Type: Conference Object
• Language: Portuguese

Description

Chrysonilia sitophila is a common mould in cork industry and has been identified as a cause of IgE sensitization and occupational asthma. This fungal species have a fast growth rate that may inhibit others species’ growth causing underestimated data from characterization of occupational fungal exposure. Aiming to ascertain occupational exposure to fungi in cork industry, were analyzed papers from 2000 about the best air sampling method, to obtain quantification and identification of all airborne culturable fungi, besides the ones that have fast-growing rates. Impaction method don’t allows the collection of a representative air volume, because even with some media that restricts the growth of the colonies, in environments with higher fungal load, such as cork industry, the counting of the colonies is very difficult. Otherwise, impinger method permits the collection of a representative air volume, since we can make dilution of the collected volume. Besides culture methods that allows fungal identification trough macro- and micro-morphology, growth features, thermotolerance and ecological data, we can apply molecular biology with the impinger method, to detect the presence of non-viable particles and potential mycotoxin producers’ strains, and also to detect mycotoxins presence with ELISA or HPLC. Selection of the best air sampling method in each setting is crucial to achieve characterization of occupational exposure to fungi. Information about the prevalent fungal species in each setting and also the eventual fungal load it’s needed for a criterious selection.