A novel flow cytometric protocol for assessment of yeast cell adhesion

• Author(s): Silva-Dias, A. ; Miranda, Isabel M. ; Rocha, R. ; Monteiro-Soares, M. ; Salvador, Alexandre ; Rodrigues, Acácio G. ; Pina-Vaz, Cidália
• Date: 2012
• Persistent ID: http://hdl.handle.net/10400.21/2458
• Origin: Repositório Científico do Instituto Politécnico de Lisboa
• Subject(s): Candida; Candida/physiology; Cell adhesion; Flow cytometry/methods; Fluorescent dyes; Microspheres; Saccharomyces cerevisiae/physiology; Cytometry
• Document Type: Article
• Language: English

Description

Microbial adhesion is a field of recognized relevance and, as such, an impressive array of tools has been developed to understand its molecular mechanisms and ultimately for its quantification. Some of the major limitations found within these methodologies concern the incubation time, the small number of cells analyzed, and the operator's subjectivity. To overcome these aspects, we have developed a quantitative method to measure yeast cells' adhesion through flow cytometry. In this methodology, a suspension of yeast cells is mixed with green fluorescent polystyrene microspheres (uncoated or coated with host proteins). Within 2 h, an adhesion profile is obtained based on two parameters: percentage and cells-microsphere population's distribution pattern. This flow cytometry protocol represents a useful tool to quantify yeast adhesion to different substrata in a large scale, providing manifold data in a speedy and informative manner.