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A novel flow cytometric protocol for assessment of yeast cell adhesion
Silva-Dias, A.; Miranda, Isabel M.; Rocha, R.; Monteiro-Soares, M.; Salvador, Alexandre; Rodrigues, Acácio G.; Pina-Vaz, CidáliaMicrobial adhesion is a field of recognized relevance and, as such, an impressive array of tools has been developed to understand its molecular mechanisms and ultimately for its quantification. Some of the major limitations found within these methodologies concern the incubation time, the small number of cells analyzed, and the operator's subjectivity. To overcome these aspects, we have developed a quantitative...
An alternative respiratory pathway on Candida krusei : implications on suscepti...
Oliveira, Sofia Costa de; Marques, Belém Sampaio; Barbosa, Matilde; Ricardo, Elisabete; Vaz, Cidália Pina; Ludovico, Paula; Rodrigues, Acácio G.Our aim was to detect the presence of an alternative oxidase (AOX) in Candida krusei clinical strains and its influence on fluconazole susceptibility and in reactive oxygen species (ROS) production. Candida krusei clinical isolates were tested to evaluate the presence of AOX. Debaromyces hansenii 2968 (AOX positive) and Saccharomyces cerevisiae BY4742 (AOX negative) were used as control strains. Measurements of...
Novel method using a laser scanning cytometer for detection of Mycobacteria in ...
Pina-Vaz, Cidália; Costa-de-Oliveira, Sofia; Rodrigues, Acácio G.; Salvador, AlexandreIn order to evaluate the capacity of laser scanning cytometry (LSC) to detect acid-fast bacilli directly on clinical samples, a comparison between Kinyoun-stained smears analyzed under light microscopy and propidium iodide-auramine-stained smears analyzed by LSC was performed. The results were compared with those for culture on BACTEC MGIT 960. LSC is a new, reliable methodology to detect Mycobacteria.
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