Novel method using a laser scanning cytometer for detection of Mycobacteria in ...

• Autor(es): Pina-Vaz, Cidália ; Costa-de-Oliveira, Sofia ; Rodrigues, Acácio G. ; Salvador, Alexandre
• Data: 2004
• Identificador Persistente: http://hdl.handle.net/10400.21/2457
• Origem: Repositório Científico do Instituto Politécnico de Lisboa
• Assunto(s): Tuberculosis; Mycobacteria; Bacilli; Bacteria; Auramine; Propidium iodide; Cytometry
• Tipo de Documento: Artigo
• Idioma: Inglês

Descrição

In order to evaluate the capacity of laser scanning cytometry (LSC) to detect acid-fast bacilli directly on clinical samples, a comparison between Kinyoun-stained smears analyzed under light microscopy and propidium iodide-auramine-stained smears analyzed by LSC was performed. The results were compared with those for culture on BACTEC MGIT 960. LSC is a new, reliable methodology to detect Mycobacteria.