Novel method using a laser scanning cytometer for detection of Mycobacteria in ...

• Author(s): Pina-Vaz, Cidália ; Costa-de-Oliveira, Sofia ; Rodrigues, Acácio G. ; Salvador, Alexandre
• Date: 2004
• Persistent ID: http://hdl.handle.net/10400.21/2457
• Origin: Repositório Científico do Instituto Politécnico de Lisboa
• Subject(s): Tuberculosis; Mycobacteria; Bacilli; Bacteria; Auramine; Propidium iodide; Cytometry
• Document Type: Article
• Language: English

Description

In order to evaluate the capacity of laser scanning cytometry (LSC) to detect acid-fast bacilli directly on clinical samples, a comparison between Kinyoun-stained smears analyzed under light microscopy and propidium iodide-auramine-stained smears analyzed by LSC was performed. The results were compared with those for culture on BACTEC MGIT 960. LSC is a new, reliable methodology to detect Mycobacteria.