Author(s):
Santos, António Mário 
; Oleastro, Mónica ; Vale de Gato, Inês ; Lopes, Teresa ; Seixas, Elsa ; Machado, Jorge ; Guerreiro, António Sousa
Date: 2011
Persistent ID: http://hdl.handle.net/10400.18/363
Origin: Repositório Científico do Instituto Nacional de Saúde
Subject(s): Helicobacter pylori; Nutraceuticals; Infecções Gastrointestinais
Description
Background and Aim: H. pylori induces severe gastric chronic inflammation
and is the cause of gastritis, peptic ulcer and a major risk factor for gastric cancer.
The aim of the study was to investigate the anti-inflammatory effect of two
nutraceuticals in Hp-infected mucosa.
Materials and Methods: Eighteen C57BL/6 mice were inoculated with Hp SS1
by gavage three times with 3 · 109 viable cells. Mice were then treated with
either PBS, curcumin (10 mg/mouse) or Symbiotic 2000 (50 mg/mouse), three
times per week. Half of the infected and three non-infected mice were euthanized
at week 6, the remaining at week 18. Gastric samples were removed for immunohistochemistry
and PCR array (inflammatory response and immunity pathway)
analysis (Sabiosciences, Qiagen).
Results: All the 18 mice were Hp positive by immunohistochemistry. The production
of the chemokines CCL2, CCL5, CCL20, CCL25, CXCL1 and CXCL11 was
significantly up-regulated at both week 6 (range of fold-change 4.3–718) and
week 18 (range of fold-change 16–1192). Similarly, the expression of the proinflammatory
cytokines IL-1b, IL6, IL9, IL10, IL23, TNFa and INFc was significantly
augmented (range of fold-change 1338–8251). The treatment with either curcumin
or symbiotic drastically decreased the expression of all these mediators,
restoring their levels to those similar to the non-infected mice.
Conclusions: The present study confirmed that Hp infection induces a strong
inflammatory response. Curcumin and Symbiotic treatments exerted a significant
anti-inflammatory effect in Hp-infected mucosa.
The supplementation of diet with these nutraceuticals may be a novel clinical
approach against gastric inflammation induced by Hp infection.
