Document details

Characterisation of polyphenols by HPLC-PAD-ESI/MS and antioxidant activity in ...

Author(s): Correia, Helena cv logo 1 ; González-Paramás, Ana cv logo 2 ; Amaral, Maria Teresa cv logo 3 ; Santos-Buelga, Celestino cv logo 4 ; Batista, Maria Teresa cv logo 5

Date: 2005

Persistent ID: http://hdl.handle.net/10316/8378

Origin: Estudo Geral - Universidade de Coimbra


Description
The antioxidant activity of an aqueous extract (infusion) and respective ethyl acetate fraction of Equisetum telmateia Ehrh. (Equisetaceae), a plant used in traditional medicine for its anti-inflammatory and diuretic properties, has been evaluated by DPPH, TEAC and TBARS assays. A high and significant antioxidant activity was detected in the ethyl acetate fraction. Analysis of the aqueous extract and the ethyl acetate fraction by HPLC-PAD-ESI/MS allowed the identification of the major phenolic compounds as flavan-3-ol, kaempferol and phenolic acid derivatives. Among the flavan-3-ols, A-type proanthocyanidins and afzelechin derivatives were detected as well as the more common B-type procyanidins, B2 and C1, whose identification was further confirmed by HPLC using detection involving chemical reaction with p-dimethylamino-cinnamaldehyde. The results suggest that the anti-inflammatory activity of E. telmateia could be due, at least in part, to the presence of compounds with antioxidant activity. Copyright © 2005 John Wiley & Sons, Ltd. http://dx.doi.org/10.1002/pca.864
Document Type Article
Language English
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