Author(s):
Jones, John G. 
; Perdigoto, Rui ; Rodrigues, Tiago B. ; Geraldes, Carlos F. G. C.
Date: 2002
Persistent ID: http://hdl.handle.net/10316/8102
Origin: Estudo Geral - Universidade de Coimbra
Document details
Quantitation of absolute 2H enrichment of plasma glucose by 2H NMR analysis of ...
Description
A simple 2H NMR method for quantifying absolute 2H-enrichments in all seven aliphatic positions of glucose following its derivatization to monoacetone glucose is presented. The method is based on the addition of a small quantity of 2H-enriched formate to the NMR sample. When the method was applied to [2-2H]monoacetone glucose samples prepared from [2-2H]glucose standards of known enrichments in the range of 0.2-2.5%, enrichment estimates derived by the NMR method were in good agreement with the real enrichment values of the [2-2H]glucose precursors. The measurement was also applied to monoacetone glucose derived from human plasma glucose samples following administration of 2H2O and attainment of isotopic steady state, where glucose H2 and body water enrichment are equivalent. In these studies, the absolute H2 enrichment of plasma glucose estimated by the formate method was in good agreement with the 2H-enrichment of body water measured by an independent method. Magn Reson Med 48:535-539, 2002. © 2002 Wiley-Liss, Inc. http://dx.doi.org/10.1002/mrm.10234
A simple 2H NMR method for quantifying absolute 2H-enrichments in all seven aliphatic positions of glucose following its derivatization to monoacetone glucose is presented. The method is based on the addition of a small quantity of 2H-enriched formate to the NMR sample. When the method was applied to [2-2H]monoacetone glucose samples prepared from [2-2H]glucose standards of known enrichments in the range of 0.2-2.5%, enrichment estimates derived by the NMR method were in good agreement with the real enrichment values of the [2-2H]glucose precursors. The measurement was also applied to monoacetone glucose derived from human plasma glucose samples following administration of 2H2O and attainment of isotopic steady state, where glucose H2 and body water enrichment are equivalent. In these studies, the absolute H2 enrichment of plasma glucose estimated by the formate method was in good agreement with the 2H-enrichment of body water measured by an independent method. Magn Reson Med 48:535-539, 2002. © 2002 Wiley-Liss, Inc. http://dx.doi.org/10.1002/mrm.10234
Document Type
Article
Language English
Language English
| Financiadores do RCAAP | |||||||
|
|
|
|
|
|
||