Decreased ANT content in Zucker fatty rats: Relevance for altered hepatic mitoc...

• Author(s): Teodoro, João ; Rolo, Anabela P. ; Oliveira, Paulo J. ; Palmeira, Carlos M.
• Date: 2006
• Persistent ID: http://hdl.handle.net/10316/5349
• Origin: Estudo Geral - Universidade de Coimbra
• Subject(s): Non-alcoholic fatty liver; Mitochondrion; Oxidative phosphorylation
• Document Type: Article
• Language: English

Description

Mitochondria from Zucker fatty (ZF) rats (a model for fatty liver disease) showed a delay in the repolarization after a phosphorylative cycle and a decrease on state 3 respiration, suggesting alterations at the phosphorylative system level. The ATPase activity showed no differences between control and ZF rats, implying alterations in other components of the phosphorylative system. A pronounced depletion in the content of the adenine nucleotide translocator (ANT) was observed by Western blotting, while no alterations were found in the mitochondrial voltage-dependent anion channel content. These data suggest that hepatic accumulation of fat impairs mitochondrial function, reflecting the loss of oxidative phosphorylation capacity caused by a decrease in the ANT content. http://www.sciencedirect.com/science/article/B6T36-4JDVM3N-3/1/6ab136a93292166e1e8c351ee22e4cdb