Development of electrochemical oxidase biosensors based on carbon nanotube-modi...

• Autor(es): Gouveia-Caridade, Carla ; Pauliukaite, Rasa ; Brett, Christopher M.A.
• Data: 2008
• Identificador Persistente: http://hdl.handle.net/10316/5285
• Origem: Estudo Geral - Universidade de Coimbra
• Assunto(s): Multi-walled carbon nanotubes; Carbon film electrodes; Glucose oxidase; Alcohol oxidase; Electrochemical biosensor
• Tipo de Documento: Artigo
• Idioma: Inglês

Descrição

Functionalised multi-walled carbon nanotubes (MWCNTs) were cast on glassy carbon (GC) and carbon film electrodes (CFE), and were characterised electrochemically and applied in a glucose-oxidase-based biosensor. MWCNT-modified carbon film electrodes were then used to develop an alcohol oxidase (AlcOx) biosensor, in which AlcOx-BSA was cross-linked with glutaraldehyde and attached by drop-coating. The experimental conditions, applied potential and pH, for ethanol monitoring were optimised, and ethanol was determined amperometrically at -0.3 V vs. SCE at pH 7.5. Electrocatalytic effects of MWCNT were observed with respect to unmodified carbon film electrodes. The sensitivity obtained was 20 times higher at carbon film/MWCNT-based biosensors than without MWCNT. http://www.sciencedirect.com/science/article/B6TG0-4RPM7TX-1/1/c7c201831d528c29b469d52592bc6561