Voltammetric and impedance studies of inosine-5'-monophosphate and hypoxanthine

• Author(s): Oliveira-Brett, Ana Maria ; Silva, Luís A. ; Farace, Giosi ; Vadgama, Pankaj ; Brett, Christopher M. A.
• Date: 2003
• Persistent ID: http://hdl.handle.net/10316/5165
• Origin: Estudo Geral - Universidade de Coimbra
• Subject(s): Inosine 5'-monophosphate; Hypoxanthine; Electrochemical adsorption; Differential pulse voltammetry; Electrochemical impedance; DNA-biosensor
• Document Type: Article
• Language: English

Description

The oxidation mechanism and adsorption of inosine 5'-monophosphate and hypoxanthine were investigated in solutions of different pH using voltammetric and impedance methods at glassy carbon electrodes. For both compounds, the pH dependence from differential pulse voltammetry showed that the same number of electrons and protons are involved in the rate-determining step of the electrochemical reaction. In the case of hypoxanthine, it was also possible to study the effect of different concentrations. At high concentrations of hypoxanthine, two oxidation peaks were observed, the first due to hypoxanthine oxidation with formation of oligomers and the second due to hypoxanthine oligomer oxidation, both compounds adsorbing strongly. Impedance measurements corroborated the voltammetric results and enabled the study of the adsorption of hypoxanthine on glassy carbon. http://www.sciencedirect.com/science/article/B6W72-47R9YPB-1/1/a6995627364cc875adcae0c8f04fbcd5