Detection of the damage caused to DNA by a thiophene-S-oxide using an electroch...

• Author(s): Brett, Ana Maria Oliveira ; Silva, Luis Antônio da ; Fujii, Hideki ; Mataka, Shuntaro ; Thiemann, Thies
• Date: 2003
• Persistent ID: http://hdl.handle.net/10316/5160
• Origin: Estudo Geral - Universidade de Coimbra
• Subject(s): Thiophene-S-oxide; DNA-biosensor; Oxidative damage; 8-Oxoguanine; Voltammetry; Electrochemistry; Oxidation; Reduction; DNA-electrochemical biosensors
• Document Type: Article
• Language: English

Description

The electrochemistry of 3,4-dibenzyl-2,5-dimethylthiophene-S-oxide was investigated as well as its interaction with dsDNA. The electrochemical study of the thiophene-S-oxide was performed at different pH values, in a mixed solvent and by solid state voltammetry, and showed that the molecule is reduced at a very negative potential, and the reduction is pH dependent. A glassy carbon electrode modified with a thick thiophene-S-oxide+dsDNA mixture was used to study the interaction of thiophene-S-oxide in situ with dsDNA at a charged interface. The experimental results give strong evidence that the reduced thiophene-S-oxide interacts with dsDNA causing damage with possible strand break and that the thiophene-S-oxide adduct formed with dsDNA can still undergo reduction. http://www.sciencedirect.com/science/article/B6TGB-48R1X8W-4/1/27fc80a2560eca0f5280d7b80cebe4a6