The DNA extraction from the pulp dentine complex of both with and without carious

• Author(s): Corte-Real, A. ; Andrade, L. ; Anjos, M. J. ; Carvalho, M. ; Vide, M. C. ; Corte-Real, F. ; Vieira, D. N.
• Date: 2006
• Persistent ID: http://hdl.handle.net/10316/4746
• Origin: Estudo Geral - Universidade de Coimbra
• Subject(s): Pulp-dentine-complex; Forensic DNA
• Document Type: Article
• Language: English

Description

The main objective of this paper is to show that, despite some adverse forensic condition such as degraded human body remains and exhumed material, the dentine (in pulp dentine complex) keeps, in the majority of cases, its integrity. In this study, we use a sample of 30 human teeth (both with and without carious) after extraction during dental treatment. We analyze 15 STRs and both high-variable regions I and II of mitochondrial DNA. Each tooth was prepared using a technique that comprises the mechanic removal of the enamel, central pulp and cement. The DNA extraction was carried out with a commercial kit, but the protocol was adjusted according to the specificities of the sample. This procedure has allowed us to obtain a genetic profile of mitochondria DNA in all the samples as well as to define a profile of STRs in some of them. http://www.sciencedirect.com/science/article/B7581-4JSBXBX-8G/1/3f71df7c58cfd5c67f909711f9af9d98