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The DNA extraction from the pulp dentine complex of both with and without carious
Corte-Real, A.; Andrade, L.; Anjos, M. J.; Carvalho, M.; Vide, M. C.; Corte-Real, F.; Vieira, D. N.The main objective of this paper is to show that, despite some adverse forensic condition such as degraded human body remains and exhumed material, the dentine (in pulp dentine complex) keeps, in the majority of cases, its integrity. In this study, we use a sample of 30 human teeth (both with and without carious) after extraction during dental treatment. We analyze 15 STRs and both high-variable regions I and I...
Haplotype frequency of the loci DYS437, DYS438, DYS439, DYS460 (Y-GATA A7.1), D...
Santos, M. V.; Carvalho, M.; Lopes, V.; Vide, M. C.; Corte-Real, F.; Vieira, D. N.The study of the Y-chromosome is very important for forensic and population genetics. Although many populations have already been analysed, there is little data about Brazilian populations. The haplotype frequency of eight Y-STRs: DYS437, DYS438, DYS439, DYS460 (Y-GATA-A7.1), DYS461 (Y-GATA-A7.2), Y-GATA-A10, Y-GATA-C4 and Y-GATA-H4 in 72 male individuals from Bahia was established. A total of 67 different hapl...
Y-chromosome STRs DYS385, DYS19, DYS389-I and II, DYS390, DYS391, DYS392 and DY...
Lopes, V.; Carvalho, M.; Antunes, S.; Anjos, M. J.; Andrade, L.; Santos, M. V.; Corte-Real, F.; Vieira, D. N.; Gamero, J. J.; Vide, M. C.Background: The Y chromosome has been used to compare the relationship between populations, representing a rich source of potential information to trace paternal lineages and providing a record of our relatedness. Among different population groups, African populations seem to be very interesting to study, considering the theory of the origin of modern humans and the ethnic variability usually existing. Methods:...
Y-chromosome DNA haplotypes in human samples from Bahia, Brazil
Santos, M. V.; Carvalho, M.; Anjos, M. J.; Andrade, L.; Lopes, V.; Corte-Real, F.; Vieira, D. N.; Vide, M. C.Background: The Y-chromosome polymorphisms have a great value to population genetic studies and evolutionary aspects since these markers show high levels of heterogeneity within and between populations, permitting the geographical distribution and ancestry of patrilineages to be evaluated. They complement the autosomal STRs and mtDNA matrilineage information. Y-linked STRs remain stable in given paternal lineag...
Y-chromosomal haplogroups in male identification: study of a population sample ...
Andrade, L.; Balsa, F.; Lopes, V.; Anjos, M. J.; Carvalho, M.; Corte-Real, F.; Vieira, D. N.; Vide, M. C.Background: The non-recombining portion of the Y chromosome represents a valuable tool for the study of human population history. The haploid, non-crossing-over behaviour of Y chromosome gives potentials in many aspects resembling the maternally inherited mtDNA. The advantages for forensic casework are obvious. The haploid state makes mixture patterns much simpler; in female/male stain mixtures, pure male DNA p...
mtDNA analysis in Portuguese populations (Central Portugal and Azores Islands):...
Carvalho, M.; Mendes, C.; Antunes, H.; Anjos, M. J.; Andrade, L.; Lopes, V.; Vieira, D. N.; Vide, M. C.Background: The polymorphism of the two hypervariable segments (HVI and HVII) of the control region of mtDNA was analyzed in a population of 81 unrelated individuals from Central Portugal and 48 from the Azores Islands, using a fluorescent-based electrophoresis sequencing method. Methods: Sequences have been obtained with ABI PRISM0 Big Dye Terminator and dRhodamine Terminator Cycle Sequencing Ready Reaction Ki...
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