Author(s):
Rocha, J. M. S. 
; Gil, M. H. ; Garcia, F. A. P.
Date: 1998
Persistent ID: http://hdl.handle.net/10316/3843
Origin: Estudo Geral - Universidade de Coimbra
Document details
Effects of additives on the activity of a covalently immobilised lipase in orga...
Description
Lipase from Mucor miehei was covalently immobilised onto the graft copolymer poly(ethylene)-g.co-hydroxyethyl methacrylate (PE-g.co-HEMA), partially hydrolysed, via a spacer arm of 1,6-diaminohexane activated with glutaraldehyde. To improve the lipolytic activity of the immobilised lipase (for the synthesis of isoamyl-caprylate, as a model), the effect of several additives was investigated. Polyethylene glycol (PEG), glutaraldehyde, organic solvents and buffers, were added during the immobilisation procedure and their effects are reported and compared with the behaviour of the lipolytic preparation without pre-treatment. An increase of 40-100% in the activity was obtained when small quantities of PEG 2000 and glutaraldehyde (used also as an activator of the spacer arm) were added. The activity had a maximum when the pH of the lipase attachment solution was 7.2 and buffered with phosphate. The effect of the aggregation level of biocatalyst particles on the amount of water retained, as well as the effect of the immobilisation on solid supports on the stability to organic solvents, is also reported. http://www.sciencedirect.com/science/article/B6T3C-3V8CG7H-7/1/f8c9c7095fea93b2ccae17922a1f89d6
Lipase from Mucor miehei was covalently immobilised onto the graft copolymer poly(ethylene)-g.co-hydroxyethyl methacrylate (PE-g.co-HEMA), partially hydrolysed, via a spacer arm of 1,6-diaminohexane activated with glutaraldehyde. To improve the lipolytic activity of the immobilised lipase (for the synthesis of isoamyl-caprylate, as a model), the effect of several additives was investigated. Polyethylene glycol (PEG), glutaraldehyde, organic solvents and buffers, were added during the immobilisation procedure and their effects are reported and compared with the behaviour of the lipolytic preparation without pre-treatment. An increase of 40-100% in the activity was obtained when small quantities of PEG 2000 and glutaraldehyde (used also as an activator of the spacer arm) were added. The activity had a maximum when the pH of the lipase attachment solution was 7.2 and buffered with phosphate. The effect of the aggregation level of biocatalyst particles on the amount of water retained, as well as the effect of the immobilisation on solid supports on the stability to organic solvents, is also reported. http://www.sciencedirect.com/science/article/B6T3C-3V8CG7H-7/1/f8c9c7095fea93b2ccae17922a1f89d6
Document Type
Article
Language English
Language English
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