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Natural immobilisation of microorganisms for continuous ethanol production
Baptista, C. M. S. G.; Cóias, J. M. A.; Oliveira, A. C. M.; Oliveira, N. M. C.; Rocha, J. M. S.; Dempsey, M. J.; Lannigan, K. C.; Benson, P. S.Using a growth medium based on cane blackstrap molasses, we compared ethanol production by two strains of Saccharomyces cerevisiae that were immobilized in polyurethane foam cubes in a fluidised-bed fermenter. One strain (NCYC 1119) was adhesive and extremely flocculent, whilst the other strain was not adhesive and only weakly flocculent. The strong flocs of NCYC 1119 caused blockage of the bed, so that stable ...
Selective extraction of carotenoids from the microalga Dunaliella salina with r...
Hejazi, M. A.; Lamarliere, C. de; Rocha, J. M. S.; Vermuë, M.; Tramper, J.; Wijffels, R. H.Simultaneous production and selective extraction of β-carotene from living cells of in a two-phase system of aqueous and organic phases has been investigated. Solvents with values of log Poctanol, which denotes hydrophobicity of a compound, ranging from 3 to 9 were used as organic phase. Viability and activity of in the presence of organic solvents were checked by microscopic observation and photosynthetic ox...
Optimisation of the enzymatic synthesis of n-octyl oleate with immobilised lipa...
Rocha, J. M. S.; Gil, M. H.; Garcia, F. A. P.The enzymatic synthesis of n-octyl oleate by direct esterification of the oleic acid and the octanol in a solvent-free medium was previously shown to be efficiently catalysed by a lipase from Rhizomucor miehei covalently linked to a graft copolymer, the partially hydrolysed poly(ethylene)-g co-hydroxyethyl methacrylate (PE-g co-HEMA). In this work we went further towards an optimisation of the production of n-o...
Effects of additives on the activity of a covalently immobilised lipase in orga...
Rocha, J. M. S.; Gil, M. H.; Garcia, F. A. P.Lipase from Mucor miehei was covalently immobilised onto the graft copolymer poly(ethylene)-g.co-hydroxyethyl methacrylate (PE-g.co-HEMA), partially hydrolysed, via a spacer arm of 1,6-diaminohexane activated with glutaraldehyde. To improve the lipolytic activity of the immobilised lipase (for the synthesis of isoamyl-caprylate, as a model), the effect of several additives was investigated. Polyethylene glycol ...
Preparation and characterization of polyethylene based graft copolymers. Applic...
Ferreira, L. M.; Rocha, J. M. S.; Andrade, M. E.; Gil, M. H.In the last few years new copolymeric supports for the immobilization of biological compounds have been developed. The graft copolymer polyethylene-g.co-hydroxyethyl methacrylate, partially hydrolyzed, has shown to be a very promising support for this purpose. ; http://www.sciencedirect.com/science/article/B6TVT-3TDH4Y8-1V/1/f6ebad7ead1ae71b48e454508d1e6e5f
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