Author(s):
Pita, T 
; Alves-Pereira, I ; Ferreira, Rui
Date: 2012
Persistent ID: http://hdl.handle.net/10174/7401
Origin: Repositório Científico da Universidade de Évora
Subject(s): organochlorine; persistent insecticide
Description
Lindane is an organochlorine persistent insecticide, lipophilic, chemically and biochemically stable, detected in the atmosphere, groundwater, sediments and soil. The literature describes this compound as toxic and able to affect animal reproduction and development. The aim of this study was to evaluate the effects of lindane in the wild-type S. cerevisiae UE-ME3 of Alentejo, Portugal, a unicellular eukaryotic organism, described as resistant to the presence of pesticides or metals. Cells at mid-exponential phase were inoculated in YEPD medium with 2% (w/v) glucose and incubated during 72 h in a water bath with orbital shaking, at 28 ºC, in the absence or in presence of 5 and 50 μM lindane. Samples from each treatment were used to obtain growth curves and to prepare post-12000 g supernatant, used for determination of glutathione and MDA content by fluorimetry as well as GR and G6PD activities by spectrophotometry. The exposure to lindane caused a shift in growth profile after 24 h of culture, occurring a decrease of cell growth in the final of exponential phase and at stationary phase, indicators of growth disturbance caused by this xenobiotic. Cells grown in presence of lindane showed an increase of G6PD and GR activities proportional to the organochlorine level in the media (r = 0.972 and r = 0.988, respectively). This response seems explain, in part, the increase in glutathione reducing power detected in post-12000 g supernatant of 50 µM lindane, as well as the absence of significant changes in cytoplasmic MDA level. The increase in the G6PD and GR activities may also be correlated with the excretion of insecticide to the vacuole or with the transference of reducing equivalents which assist to anabolic pathways involved in the maintenance of cell proliferation. Despite the harmful effects caused by lindane, the reducing power transferred by G6PD and GR enzymes appear to be sufficient to minimize cell damages in the cytoplasm of S. cerevisiae UE-ME3. The cell growth disruption can eventualy results from events at peroxisome and/or mitochondria.
