Impact of tillage system on arbuscular mycorrhiza fungal communities in the soi...

• Author(s): Brito, Isabel ; Goss, Michael J. ; Carvalho, Mário ; Chatagnier, Odile ; van Tuinen, Diederich
• Date: 2012
• Persistent ID: http://hdl.handle.net/10174/6828
• Origin: Repositório Científico da Universidade de Évora
• Subject(s): Arbuscular mycorrhiza (AM) fungi; Genetic diversity; Soil tillage; Large ribosomal subunit (LSU rDNA)
• Document Type: Article
• Language: Portuguese

Description

A more diverse arbuscular mycorrhiza (AM) fungal community should be more versatile and resilient to variation in environmental conditions over space and time. To evaluate the effect of no-till and conventional tillage systems, AM fungal diversity was assessed as part of a long term field experiment by sequencing of DNA, extracted from soil, that encoded the large ribosomal sub-unit and was obtained by nested-PCR. In comparison with no-till, conventional tillage decreased AM fungal diversity by 40%. Differences between treatments in the frequency of the operational taxonomic units (OTUs) present in soil, confirm that AM fungi are differently vulnerable to soil disturbance.