High diversity of chickpea Mesorhizobium species isolated in a Portuguese agric...

• Author(s): Laranjo, Marta ; Machado, Jorge ; Young, J.Peter W. ; Oliveira, Solange
• Date: 2004
• Persistent ID: http://hdl.handle.net/10174/2105
• Origin: Repositório Científico da Universidade de Évora
• Subject(s): mesorhizobium; diversity
• Document Type: Article
• Language: English

Description

Chickpea rhizobia isolated from Portuguese soils were assigned to the genus Mesorhizobium by 16S-rDNA sequencing. High species diversity was found within populations of an agricultural region in the south of Portugal.;Besides the expected Mesorhizobium ciceri and M. mediterraneum, some isolates were close to M. loti or M. tianshanense and some formed a clade that may represent a new species. A new PCR-based approach, named direct amplified polymorphic DNA (DAPD) analysis, supported the 16S-based phylogeny. This suggests that this method could be used as a molecular tool to assess genetic relationships. Evaluation of genetic diversity by 16S-rDNA sequence, DAPD and protein profiles showed different levels of heterogeneity in natural populations