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Legume growth-promoting rhizobia: An overview on the Mesorhizobium genus
Laranjo, Marta; Alexandre, Ana; Oliveira, SolangeThe need for sustainable agricultural practices is revitalizing the interest in biological nitrogen fixationand rhizobia-legumes symbioses, particularly those involving economically important legume crops interms of food and forage. The genus Mesorhizobium includes species with high geographical dispersionand able to nodulate a wide variety of legumes, including important crop species, like chickpea or bis-erru...
Global Transcriptional Response to Heat Shock of the Legume Symbiont Mesorhizob...
Alexandre, Ana; Laranjo, Marta; Oliveira, SolangeRhizobia, the bacterial legume symbionts able to fix atmospheric nitrogen inside root nodules, have to survive in varied environmental conditions. The aim of this study was to analyse the transcriptional response to heat shock ofMesorhizobium lotiMAFF303099, a rhizobium with a large multipartite genome of 7.6 Mb that nodulates the model legume Lotus japonicus. Using microarray analysis, extensive transcriptomic...
Molecular characterization of Portuguese populations of the pinewood nematode B...
Valadas, Vera; Laranjo, Marta; Mota, Manuel; Oliveira, SolangeBursaphelenchus xylophilus is the causal agent of pine wilt disease and a worldwide pest with high economic impact. Since its first diagnosis in Portugal in 1999, it has been subjected to quarantine measures with impact on forest health and ecosystem stability, significantly affecting international trade of wood products. The disease was detected in the north and centre of continental Portugal and, since 2008, ...
A survey of entomopathogenic nematode species in continental Portugal
Valadas, Vera; Laranjo, Marta; Mota, ManuelEntomopathogenic nematodes (EPN) are lethal parasites of insects, used as biocontrol agents. The objectives of thisworkwere to survey the presence of EPNin continental Portugal and to characterize the different species. Of the 791 soil samples collected throughout continental Portugal, 53 were positive for EPN. Steinernema feltiae and Heterorhabditis bacteriophora were the two most abundant species. Analysis of...
Multilocus sequence analysis reveals multiple symbiovars within Mesorhizobium s...
Laranjo, Marta; Young, J. Peter W.; Oliveira, SolangeThe genus Mesorhizobium includes species nodulating several legumes, such as chickpea, which has a high agronomic importance. Chickpea rhizobia were originally described as either Mesorhizobium ciceri or M. mediterraneum. However, rhizobia able to nodulate chickpea have been shown to belong to several different species within the genus Mesorhizobium. The present study used a multilocus sequence analysis approac...
The pine wood nematode, Bursaphelenchus xylophilus, in Portugal: possible intro...
Valadas, Vera; Laranjo, Marta; BARBOSA, Pedro; Espada, Margarida; Mota, Manuel; Oliveira, SolangeThe pine wood nematode (PWN), Bursaphelenchus xylophilus, the causal agent of pine wilt disease (PWD), is a major world-wide pathogen and pest of pine, with impacts on forest health, natural ecosystem stability and international trade. In Portugal, PWN was first diagnosed in 1999, the first occurrence also for Europe. The disease was recently detected on the island ofMadeira and in northern Spain. In an attempt...
Tolerance of Mesorhizobium type strains to different environmental stresses
Laranjo, Marta; Oliveira, SolangeThe symbiosis between rhizobia and legumes is affected by different environmental conditions. Our aims were to evaluate stress tolerance of Mesorhizobium species and investigate species-specific stress response mechanisms. Tolerance of Mesorhizobium type strains to temperature, salt and pH stress was evaluated. Mesorhizobium thiogangeticum showed highest growth with 1.5% NaCl and Mesorhizobium ciceri at pH 5. M...
Survey of Chickpea Rhizobia Diversity in Portugal Reveals the Predominance of S...
Alexandre, Ana; Brígido, Clarisse; Laranjo, Marta; Rodrigues, Sérgio; Oliveira, SolangeSeveral Mesorhizobium species are able to induce effective nodules in chickpea, one of the most important legumes worldwide. Our aims were to examine the biogeography of chickpea rhizobia, to search for a predominant species, and to identify the most efficient microsymbiont, considering Portugal as a case study. One hundred and ten isolates were obtained from continental Portugal and Madeira Island. The 16S rib...
Chickpea rhizobia symbiosis genes are highly conserved across multiple Mesorhiz...
Laranjo, Marta; Alexandre, Ana; Rivas, Raul; Velázquez, Encarna; Young, J. Peter W.; Oliveira, SolangeABSTRACT Chickpea has been considered as a restrictive host for nodulation by rhizobia. However, recent studies have reported that several Mesorhizobium species may effectively nodulate chickpea. With the purpose of investigating the evolutionary relationships between these different species with the ability of nodulating the same host, we analysed 21 Portuguese chickpea rhizobial isolates. Symbiosis genes nifH...
dnaJ is a useful phylogenetic marker for alphaproteobacteria
Alexandre, Ana; Laranjo, Marta; Young, J. Peter W.; Oliveira, SolangeSummary In the past, bacterial phylogeny relied almost exclusively on 16S rRNA gene sequence analysis. More recently, multilocus sequences have been used to infer organismal phylogenies. In this study, the dnaJ chaperone gene was investigated as a marker for phylogeny studies in alphaproteobacteria. Preliminary analysis of G+C and G+C3s contents showed no clear evidence of horizontal transfer of this gene in...
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