Autor(es):
Alexandre, Ana 
; Laranjo, Marta ; Young, J. Peter W. ; Oliveira, Solange
Data: 2008
Identificador Persistente: http://hdl.handle.net/10174/1371
Origem: Repositório Científico da Universidade de Évora
Assunto(s): alphaproteobacteria; DnaJ phylogeny
Descrição
Summary
In the past, bacterial phylogeny relied almost exclusively on 16S rRNA gene sequence analysis. More recently, multilocus sequences have been used to infer organismal phylogenies. In this study, the dnaJ chaperone gene was investigated as a marker for phylogeny studies in alphaproteobacteria. Preliminary analysis of G+C and G+C3s contents showed no clear evidence of horizontal transfer of this gene in proteobacteria. dnaJ-based phylogenies were then analysed at three taxonomic levels: Proteobacteria, Alphaproteobacteria and the genus Mesorhizobium. Dendrograms based on DnaJ and 16S rRNA gene sequences revealed the same topology already described for Proteobacteria. These results indicate that the DnaJ phylogenetic signal is able to reproduce the accepted relationships among the five Proteobacteria classes. At a lower taxonomic level, using 20 alphaproteobacteria species, the 16S rRNA gene-based phylogeny is distinct from the one based on the DnaJ sequence analysis. Although the same clusters are generated, only the topology of the DnaJ tree is consistent with broader phylogenies from recent studies based on concatenated alignments of multiple core genes. For example, the DnaJ tree shows the two Rhizobiales clusters, closely related, as expected, while the 16S rRNA gene-based phylogeny shows them distantly related. In order to evaluate the phylogenetic performance of dnaJ at the genus level, a multilocus analysis based on five housekeeping genes (atpD, gapA, gyrB, recA, rplB) was performed for ten Mesorhizobium species. In contrast to the 16S rRNA gene, the DnaJ sequence analysis generated a tree similar to the multilocus dendrogram. For identification of chickpea mesorhizobium isolates, a dnaJ nucleotide-based tree was used. Despite different topologies, 16S rRNA gene- and dnaJ-based trees led to the same species identification. This study suggests that the dnaJ gene is a good phylogenetic marker, particularly for the Alphaproteobacteria class, since its phylogeny is consistent with phylogenies based on multilocus approaches.
